期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 24, 期 7, 页码 1016-1025出版社
AMER CHEMICAL SOC
DOI: 10.1007/s13361-013-0644-7
关键词
Hydrogen/deuterium exchange; Allergen; Cashew; Epitope mapping; FTMS
资金
- NIH [R01 GM78359]
- NSF Division of Materials Research [DMR-06-54118]
- USDA NIFA-AFRI [2010-04213]
- Almond Board of California
- State of Florida
The potential epitopes of a recombinant food allergen protein, cashew Ana o 2, reactive to polyclonal antibodies, were mapped by solution-phase amide backbone H/D exchange (HDX) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Ana o 2 polyclonal antibodies were purified in the serum from a goat immunized with cashew nut extract. Antibodies were incubated with recombinant Ana o 2 (rAna o 2) to form antigen:polyclonal antibody (Ag:pAb) complexes. Complexed and uncomplexed (free) rAna o 2 were then subjected to HDX-MS analysis. Four regions protected from H/D exchange upon pAb binding are identified as potential epitopes and mapped onto a homologous model.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据