期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 24, 期 5, 页码 801-804出版社
SPRINGER
DOI: 10.1007/s13361-013-0586-0
关键词
para-Nitroaniline; MALDI-MSI; Mass spectrometry imaging; Intermediate pressure; MALDI
资金
- EPSRC through the PSIBS Doctoral Training Centre at the University of Birmingham [EP/F50053X/1]
- EPSRC [EP/F50053X/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [964940] Funding Source: researchfish
para-Nitroaniline (PNA) is presented as a promising matrix for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) on an intermediate-pressure ion source (1 Torr) QqTOF instrument using an Nd:YVO4 laser operated at 5 kHz. An imaging study was carried out to determine the utility of PNA at this pressure by analyzing 14 tissue sections. We demonstrate acquisition of high-quality imaging data over a 6-h period in the ion source. In this study, comparisons were made between PNA and alpha-cyano-4-hydroxycinnamic acid (CHCA) in positive ion mode to demonstrate the utility of PNA in these circumstances. PNA performed as well as or better than CHCA in terms of lipid ion intensities, resulting in lower levels of ion fragmentation and in lower incidences of analyte migration at the edges of the tissue sections when using airspray matrix deposition.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据