期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 23, 期 3, 页码 442-445出版社
SPRINGER
DOI: 10.1007/s13361-011-0320-8
关键词
Inlet ionization; Nano-solvent assisted inlet ionization (nSAII); Liquid chromatography (LC); Mass spectrometry (MS); data dependent MS/MS; BSA tryptic digest; Nano-electrospray ionization (nESI)
资金
- NSF [0955975]
- ASMS (Waters Company)
- DuPont Young Professor Award
- Schaap Fellowship
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0955975] Funding Source: National Science Foundation
Liquid chromatography (LC) solvent assisted inlet ionization (SAII) mass spectrometry (MS) was previously reported to give good chromatographic resolution and MS detection injecting 66 ng of a BSA tryptic digest. In analogy to nano-electrospray ionization (nESI), we extend SAII LC/MS to nano-SAII (nSAII) operating at nL min(-1) flow rates and demonstrate good quality ion chromatograms and mass spectra from injection of as little as 0.7 ng of BSA digest onto a capillary LC column. Data dependent fragmentation is demonstrated for injection of 7 ng of a BSA digest. This method has advantages over nESI in ease of use and low cost as it requires no voltage and is operational without the necessity of connectors or fragile nESI emitters, although similar constricted tips can be helpful in nSAII to stabilize the signal at low nanoliter flow. At a flow rate of 0.8 mu L min(-1), the only requirement for nSAII is that the exit-end of the capillary LC column be adjusted near the aperture of the heated inlet of the mass spectrometer.
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