Enzyme-Assisted Aqueous Extraction of Kalahari Melon Seed Oil: Optimization Using Response Surface Methodology

Enzymatic extraction of oil from Kalahari melon seeds was investigated and evaluated by response surface methodology (RSM). Two commercial protease enzyme products were used separately: Neutrase(A (R)) 0.8 L and Flavourzyme(A (R)) 1000 L from Novozymes (Bagsvaerd, Denmark). RSM was applied to model and optimize the reaction conditions namely concentration of enzyme (20-50 g kg(-1) of seed mass), initial pH of mixture (pH 5-9), incubation temperature (40-60 A degrees C), and incubation time (12-36 h). Well fitting models were successfully established for both enzymes: Neutrase 0.8 L (R (2) = 0.9410) and Flavourzyme 1000 L (R (2) = 0.9574) through multiple linear regressions with backward elimination. Incubation time was the most significant reaction factor on oil yield for both enzymes. The optimal conditions for Neutrase 0.8 L were: an enzyme concentration of 25 g kg(-1), an initial pH of 7, a temperature at 58 A degrees C and an incubation time of 31 h with constant shaking at 100 rpm. Centrifuging the mixture at 8,000g for 20 min separated the oil with a recovery of 68.58 +/- A 3.39%. The optimal conditions for Flavourzyme 1000 L were enzyme concentration of 21 g kg(-1), initial pH of 6, temperature at 50 A degrees C and incubation time of 36 h. These optimum conditions yielded a 71.55 +/- A 1.28% oil recovery.