期刊
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
卷 57, 期 25, 页码 2516-2526出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jacc.2011.02.036
关键词
atherosclerosis; inflammation; intravascular imaging; molecular imaging; stent
资金
- National Institutes of Health [R01 HL 108229, R01 HL 80472]
- American Heart Association [0830352N]
- Howard Hughes Medical Institute
- Broadview Ventures
- Donald W. Reynolds Foundation
- European Community [FP7/2007-2013, 235689]
- MGH
- Abbott Vascular
Objectives This study sought to develop a 2-dimensional (2D) intravascular near-infrared fluorescence (NIRF) imaging strategy for investigation of arterial inflammation in coronary-sized vessels. Background Molecular imaging of arterial inflammation could provide new insights into the pathogenesis of acute myocardial infarction stemming from coronary atheromata and implanted stents. Presently, few high-resolution approaches can image inflammation in coronary-sized arteries in vivo. Methods A new 2.9-F rotational, automated pullback 2D imaging catheter was engineered and optimized for 360 degrees viewing intravascular NIRF imaging. In conjunction with the cysteine protease-activatable imaging reporter Prosense VM110 (VisEn Medical, Woburn, Massachusetts), intra-arterial 2D NIRF imaging was performed in rabbit aortas with atherosclerosis (n = 10) or implanted coronary bare-metal stents (n = 10, 3.5-mm diameter, day 7 post-implantation). Intravascular ultrasound provided coregistered anatomical images of arteries. After sacrifice, specimens underwent ex vivo NIRF imaging, fluorescence microscopy, and histological and immunohistochemical analyses. Results Imaging of coronary artery-scaled phantoms demonstrated 8-sector angular resolution and submillimeter axial resolution, nanomolar sensitivity to NIR fluorochromes, and modest NIRF light attenuation through blood. High-resolution NIRF images of vessel wall inflammation with signal-to-noise ratios > 10 were obtained in real-time through blood, without flushing or occlusion. In atherosclerosis, 2D NIRF, intravascular ultrasound-NIRF fusion, microscopy, and immunoblotting studies provided insight into the spatial distribution of plaque protease activity. In stent-implanted vessels, real-time imaging illuminated an edge-based pattern of stent-induced arterial inflammation. Conclusions A new 2D intravascular NIRF imaging strategy provides high-resolution in vivo spatial mapping of arterial inflammation in coronary-sized arteries and reveals increased inflammation-regulated cysteine protease activity in atheromata and stent-induced arterial injury. (J Am Coll Cardiol 2011;57:2516-26) (C) 2011 by the American College of Cardiology Foundation
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