期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 37, 页码 11771-11776出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.8b07073
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资金
- National Research Foundation of Korea (NRF) grants - Korean government (MSIP) [NRF-2018R1D1A1B07044894, NRF-2017M3D9A1073769, NRF-2015R1A5A1008958]
- National Research Foundation of Korea [21A20131212408, 2018R1D1A1B07044894, 2015R1A5A1008958] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (>= 1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.
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