期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 136, 期 37, 页码 12852-12855出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja507372v
关键词
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资金
- Natural Sciences and Engineering Research Council of Canada (CREATE Biointerfaces Training Program)
- Sentinel Bioactive Paper Network
- Canada Foundation for Innovation
- Ontario Innovation Trust
The passivation of nonspecific protein adsorption to paper is a major barrier to the use of paper as a platform for microfluidic bioassays. Herein we describe a simple, scalable protocol based on adsorption and cross-linking of poly(oligoethylene glycol methacrylate) (POEGMA) derivatives that reduces nonspecific adsorption of a range of proteins to filter paper by at least 1 order of magnitude without significantly changing the fiber morphology or paper macroporosity. A lateral-flow test strip coated with POEGMA facilitates effective protein transport while also confining the colorimetric reporting signal for easier detection, giving improved performance relative to bovine serum albumin (BSA)-blocked paper. Enzyme-linked immunosorbent assays based on POEGMA-coated paper also achieve lower blank values, higher sensitivities, and lower detection limits relative to ones based on paper blocked with BSA or skim milk. We anticipate that POEGMA-coated paper can function as a platform for the design of portable, disposable, and low-cost paper-based biosensors.
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