期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 136, 期 31, 页码 11050-11056出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja5048285
关键词
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资金
- National Basic Research Program of China (973 Program) [2012CB933102]
- National Natural Science Foundation of China [NSFC 21233001, 21190034, 21172101]
- Specialized Research Fund for the Doctoral Program of Higher Education [SRFDP 20110211130001]
- Fundamental Research Funds for the Central Universities
- 111 Project
We report an electrochemically switched smart surface for controlled peptide immobilization and conformation control. This dynamic surface is based on self-assembled monolayers (SAMs) containing surface-bound trimethoxybenzene moieties, which can undergo electrochemically modulated surface activation to be stepwisely converted to two catechol derivatives. This new smart surface can be used to realize stepwise immobilization of a peptide, and more importantly, to control peptide conformation on a surface. We demonstrate herein that with one electrochemical activation step, a linear peptide containing an RGD sequence can be attached onto the SAMs. With the subsequence activation step, the attached linear RGD peptide can be converted into cyclic conformation. The SAMs bounded with linear and cyclic RGD exhibit different adhesion behaviors to fibroblasts cells. The reaction procedure can be well-monitored by cyclic voltammetry (CV), electrochemical surface enhanced Raman microscopy (EC-SERS), and X-ray photoelectron spectroscopy (XPS). It is believed this robust smart surface can find wide applications in surface immobilization of bioactive moieties.
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