期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 13, 页码 4906-4909出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja311960g
关键词
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资金
- NIH [1DP2OD008677]
- Department of Defense NDSEG Fellowship
- Burroughs Wellcome Fund
Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are lacking. We have designed RNA-based fluorescent biosensors for cyclic di-GMP and cyclic AMP-GMP by fusing the Spinach aptamer to variants of a natural GEMM-1 riboswitch. In live cell imaging experiments, these biosensors demonstrate fluorescence turn-on in response to cyclic dinucleotides, and they were used to confirm in vivo production of cyclic AMP-GMP by the enzyme DncV.
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