期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 136, 期 1, 页码 282-290出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja409515v
关键词
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资金
- National Science Foundation [CHE-0911191]
- Thomas C. Rumble Graduate Fellowship at Wayne State University
The DNA i-motif conformation was discovered in (CCG) center dot(CGG)(n) trinucleotide repeats, which are associated with fragile X syndrome, the most widespread inherited cause of mental retardation in humans. The DNA i-motif is a four-stranded structure whose strands are held together by proton-bound dimers of cytosine (C+center dot C). The stronger base-pairing interactions in C+center dot C proton-bound dimers as compared to Watson-Crick G center dot C base pairs are the major forces responsible for stabilization of i-motif conformations. Methylation of cytosine results in silencing of the FMRI gene and causes fragile X syndrome. However, the influence of methylation or other modifications such as halogenation of cytosine on the base-pairing energies (BPEs) in the i-motif remains elusive. To address this, proton-bound heterodimers of cytosine and 5-methylcytosine, 5-fluorocytosine, 5-bromocytosine, and 5-iodocytosine are probed in detail. Experimentally, the BPEs of proton-bound heterodimers of cytosine and modified cytosines are determined using threshold collision-induced dissociation (TCID) techniques. All modifications at the 5-position of cytosine are found to lower the BPE and therefore would tend to destabilize DNA i-motif conformations. However, the BPEs in these proton-bound heterodimers still significantly exceed those of the Watson-Crick G center dot C and neutral C center dot C base pairs, suggesting that C+center dot C mismatches are still energetically favored such that i-motif conformations are preserved. Excellent agreement between TCID measured BPEs and B3LYP calculated values is found with the def2-TZVPPD and 6-311+G(2d,2p) basis sets, suggesting that calculations at these levels of theory can be employed to provide reliable energetic predictions for related systems.
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