期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 47, 页码 17727-17730出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja409936r
关键词
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资金
- Ministry of Science and Technology Basic Research Program [2011CB809100]
- National Natural Science Foundation of China [21002003, 91013003, 21272016]
- Beijing Nova Program [2010B002]
- Doctoral Fund of Ministry of Education of China [20120001110083]
We report a DNA-templated synthesis method that allows construction of the entire DNA-encoded library with a single DNA template. Taking advantage of deoxyinosine's indiscriminate base-pairing property, we designed a universal template that is capable of directing chemical reactions with multiple reactant DNAs with different sequences. In combination with other design features including photocleavable linkers and direct encoding by the reactant DNA, we demonstrated the capabilities of the universal template in library synthesis, target selection, and hit decoding. Our method can be generally and straightforwardly applied to prepare a variety of chemically diverse DNA-encoded libraries.
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