期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 45, 页码 16766-16769出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja407867a
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资金
- National Institutes of Health [GM 085092]
- National Science Foundation [DBI-0923133]
The tetrazole-based photoclick chemistry has provided a powerful tool to image proteins in live cells. To extend photoclick chemistry to living organisms with improved spatiotemporal control, here we report the design of naphthalene-based tetrazoles that can be efficiently activated by two-photon excitation with a 700 nm femtosecond pulsed laser. A water-soluble, cell-permeable naphthalene-based tetrazole was identified that reacts with acrylamide with the effective two-photon cross-section for the cycloaddition reaction determined to be 3.8 GM. Furthermore, the use of this naphthalene-tetrazole for real-time, spatially controlled imaging of microtubules in live mammalian cells via the fluorogenic, two-photon-triggered photoclick chemistry was demonstrated.
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