期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 14, 页码 5320-5323出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja401494e
关键词
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资金
- NIH [R01-GM097399]
- Alfred P. Sloan Research Fellowship
- Army Research Office [62570EGII]
Studying chemomechanical coupling at interfaces is important for fields ranging from lubrication and tribology to microfluidics and cell biology. Several polymeric macro- and microscopic systems and cantilevers have been developed to image forces at interfaces, but few materials are amenable for molecular tension sensing. To address this issue, we have developed a gold nanoparticle sensor for molecular tension-based fluorescence microscopy. As a proof of concept, we imaged the tension exerted by integrin receptors at the interface between living cells and a substrate with high spatial (<1 mu m) resolution, at 100 ms acquisition times and with molecular specificity. We report integrin tension values ranging from 1 to 15 pN and a mean of similar to 1 pN within focal adhesions. Through the use of a conventional fluorescence microscope, this method demonstrates a force sensitivity that is 3 orders of magnitude greater than is achievable by traction force microscopy or polydimethylsiloxane micro-post arrays,(1) which are the standard in cellular biomechanics.
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