期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 8, 页码 2903-2906出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja311688g
关键词
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资金
- National Natural Science Foundation of China [21174022, 21173217]
- National Basic Research Program of China [2013CB733702]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110041110009]
We have developed Lyso-V, the first fluorescent probe of lysosomal viscosity. Because of its lysosome-actived fluorescence characteristics, Lyso-V has proved to be an ideal lysosomal tracer with high spatial and temporal resolution under laser confocal microscopy. More importantly, Lyso-V shows its practical applicability in real-time quantification of lysosomal viscosity changes in live cells through fluorescence lifetime imaging microscopy.
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