期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 36, 页码 14678-14681出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja306265n
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资金
- A*STAR-ANR Program
A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).
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