期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 7, 页码 3346-3348出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja2115663
关键词
-
资金
- ARO Institute for Collaborative Biotechnologies
- NIH [R01EB007689, R01EB009764]
Transcription factor expression levels, which sensitively reflect cellular development and disease state, are typically monitored via cumbersome, reagent-intensive assays that require relatively large quantities of cells. Here, we demonstrate a simple, quantitative approach to their detection based on a simple, electrochemical sensing platform. This sensor sensitively and quantitatively detects its target transcription factor in complex media (e.g., 250 mu g/mL crude nuclear extracts) in a convenient, low-reagent process requiring only 10 mu L of sample. Our approach thus appears a promising means of monitoring transcription factor levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据