期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 10, 页码 4983-4989出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja300460p
关键词
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资金
- NSF [DMR-1006546, ECS-0335765]
- Harvard MRSEC [DMR-080484]
- Center for International Cooperation at FU Berlin
- FU Berlin
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1006546, 820484] Funding Source: National Science Foundation
Micrometer-sized hydrogel particles that contain living cells can be fabricated with exquisite control through the use of droplet-based microfluidics and bioinert polymers such as polyethyleneglycol (PEG) and hyperbranched polyglycerol (hPG). However, in existing techniques, the microgel gelation is often achieved through harmful reactions with free radicals. This is detrimental for the viability of the encapsulated cells. To overcome this limitation, we present a technique that combines droplet microfluidic templating with bio-orthogonal thiol-ene click reactions to fabricate monodisperse, cell laden microgel particles. The gelation of these microgels is achieved via the nucleophilic Michael addition of dithiolated PEG macro-cross-linkers to acrylated hPG building blocks and does not require any initiator. We systematically vary the microgel properties through the use of PEG linkers with different molecular weights along with different concentrations of macromonomers to investigate the influence of these parameters on the viability and proliferation of encapsulated yeast cells. We also demonstrate the encapsulation of mammalian cells including fibroblasts and lymphoblasts.
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