期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 27, 页码 11153-11160出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja212125w
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [16370071, 16659003, 20117003, 23249004, 23113504]
- Hohansha Foundation
- FCT [SFRH/BD/15210/2004]
- Oncasym
- Programa Gulbenkian de Doutoramento em Biomedicina
- Swiss National Science Foundation
- Swiss SystemsX.ch initiative
- ERC [LipidX-2008/011]
- NCCR Chemical Biology
- Polish-Swiss research program
- Grants-in-Aid for Scientific Research [16659003, 16370071, 23249004, 20117003] Funding Source: KAKEN
- Fundação para a Ciência e a Tecnologia [SFRH/BD/15210/2004] Funding Source: FCT
Optical highlighters are photoactivatable fluorescent molecules that exhibit pronounced changes in their spectral properties in response to irradiation with light of a specific wavelength and intensity. Here, we present a novel design strategy for a new class of caged BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorophores, based on the use of photoremovable protecting groups (PRPGs) with high reduction potentials that serve as both a photosensitive unit and a fluorescence quencher via photoinduced electron transfer (PeT). 2,6-Dinitrobenzyl (DNB)-caged BODIPY was efficiently photoactivated, with activation ratios exceeding 600-fold in aqueous solutions. We then combined this photoactivatable fluorophore with a SNAP (mutant of O-6-alkylguanine DNA alkyltransferase) ligand to obtain a small-molecule-based optical highlighter for visualization of protein dynamics, using the well-established SNAP tag technology. As proof of concept, we demonstrate spatiotemporal imaging of the fusion protein of epidermal growth factor receptor (EGFR) with SNAP tag in living cells. We also demonstrate highlighting of cells of interest in live zebrafish embryos, using the fusion protein of histone 2A with SNAP tag.
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