期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 29, 页码 11988-11991出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja305665f
关键词
-
资金
- NIH [SRR0256362, AI047818, CA044848, GM096711]
- California Sea Grant Program [R/NMP-100]
- Alexander von Humboldt-Foundation
- DFG
- PhRMA Foundation
Meroterpenoids are mixed polyketide-terpenoid natural products with a broad range of biological activities. Herein, we present the structures of four new meroterpenoid antibiotics, merochlorins A-D, produced by the marine bacterium Streptomyces sp. strain CNH-189, which possess novel chemical skeletons unrelated to known bacterial agents. Draft genome sequencing, mutagenesis, and heterologous biosynthesis in the genome-minimized model actinomycete Streptomyces coelicolor provided the 57.6 kb merochlorin gene cluster that contains two genes encoding rare bacterial vanadium-dependent haloperoxidase (VHPO) genes. Pathway expression of two different fosmid clones that differ largely by the presence or absence of the VHPO gene mcl40 resulted in the differential biosynthesis of merochlorin C, suggesting that Mcl40 catalyzes an unprecedented 15-membered chloronium-induced macrocyclization reaction converting merochlorin D to merochlorin C.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据