期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 7, 页码 3326-3329出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja210304j
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资金
- Deutsche Forschungsgemeinschaft [SFB 803]
- Fonds der Chemischen Industrie
- IMPRS
- National Institutes of Health [GM065500, CA125033]
The multivalent carbohydrate-carbohydrate interaction between membrane-anchored epitopes derived from the marine sponge Microciona prolifera has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane-coated surfaces was employed to mimic native cell cell contacts. The dynamic strength of the homomeric self-association was measured as a function of calcium ions and loading rate. A deterministic model was used to estimate the basic energy landscape and number of participating bonds in the contact zone.
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