期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 49, 页码 19957-19960出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja308875r
关键词
-
资金
- NSFC [21005026, 21135001]
- DFMEC [20100151120008, 20110161110006]
- '973' National Key Basic Research Program [2011CB911000]
- Foundation for Innovative Research Groups of NSFC [21221003]
- U.S. National Institutes of Health [GM066137, GM079359, CA133086]
- China National Instrumentation Program [2011YQ03012412]
- special fund of Chongqing Key Laboratory
A DNA configuration switch is designed to fabricate a reversible and regenerable Raman-active substrate. The substrate is composed of a Au film and a hairpin-shaped DNA strand (hot-spot-generation probes, HSGPs) labeled with dye-functionalized silver nanoparticles (AgNPs). Another ssDNA that recognizes a specific trigger is used as an antenna. The HSGPs are immobilized on the Au film to draw the dye-functionalized AgNPs close to the Au surface and create an intense electromagnetic field. Hybridization of HSGP with the two arm segments of the antenna forms a triplex-stem structure to separate the dye-functionalized AgNPs from the Au surface, quenching the Raman signal. Interaction with its trigger releases the antenna from the triplex-stem structure, and the hairpin structure of the HSGP is restored, creating an effective off-on Raman signal switch. Nucleic acid sequences associated with the HIV-1 US long terminal repeat sequences and ATP are used as the triggers. The substrate shows excellent reversibility, reproducibility, and controllability of surface-enhanced Raman scattering (SERS) effects, which are significant requirements for practical SEAS sensor applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据