期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 11, 页码 5052-5055出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja300475k
关键词
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资金
- NSFC [21173078, 20903039]
- Shanghai Rising-Star Program [10QA1401600]
- Shanghai Science and Technology Commission [11JC1403300]
- Fundamental Research Funds for the Central Universities
A new concept in which only the molecular target, such as a thiol-bearing protein, can activate the phototrigger has been demonstrated. Such target-activatable phototriggers comprise three parts: a 7-aminocoumarin phototrigger, an electron acceptor (maleimide) that efficiently quenches the coumarin excited state, and a caged leaving group attached to the coumarin. In the absence of mercaptans, photoinduced electron transfer between coumarin and maleimide effectively blocks both the fluorescence and photocleavage pathways. Thiol-bearing molecules, however, readily annihilate the electron acceptor and thus restore the phototrigger for photorelease of the caged cargo (e.g., biotin). Unlike traditional phototriggers, functional-group-activated phototriggers allow easy handling under ambient light, report specific bonding to the target, and enable photocleavage capability selectively at the binding site in situ, thus effectively positioning the photoreleased cargo at the target. Meanwhile, the unique feature of thiol-specific activation of the fluorescence and photocleavage make our new phototrigger a universal tool that can be used to identify accurately protein cysteine S-nitrosylation, a physiologically important posttranslational modification.
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