期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 42, 页码 17342-17345出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja304829g
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资金
- NNSF [30900021, 20832009, 20921091]
- SRF for 39th ROCS-SEM
- 973 program [2010CB833200, 2012CB721100]
- CAS of China [KJCX2-YW-201]
The natural products pyrroindomycins (PYRs), active against various drug-resistant pathogens, possess a characteristic, cyclohexene ring spiro-linked tetramate moiety. In this study, investigation into PYR biosynthesis revealed two new proteins, both of which, phylogenetically distinct from but functionally substitutable to each other in vivo, individually catalyze a Dieckmann cyclization in vitro for converting an N-acetoacetyl-L-alanyl thioester into a tetramate. Their counterparts are commonly present in the biosynthetic pathways of spiro and polyether tetronates, supporting a uniform paradigm for tetronate/tetramate formation, which features an enzymatic way to generate the C-X (X = O or N) bond first and the C-C bond next in building of the 5-membered heterocycle.
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