期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 24, 页码 10138-10145出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja302284p
关键词
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资金
- NSF CAREER award [CHE-1055569]
- John S. Dunn Gulf Coast Consortium for Chemical Genomics Robert A. Welch Collaborative Grant Program
- Virginia and L. E. Simmons Family Foundation
- G. Harold and Leila Y. Mathers Foundation
- Robert A. Welch Foundation research grant [C-1680]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0947054] Funding Source: National Science Foundation
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1055569] Funding Source: National Science Foundation
In this study, we present advances in the use of rhodium(II) metallopeptides for protein modification. Site-specific, proximity-driven modification is enabled by the unique combination of peptide-based molecular recognition and a rhodium catalyst capable of modifying a wide range of amino-acid side chains. We explore catalysis based on coiled-coil recognition in detail, providing an understanding of the determinants of specificity and culminating in the demonstration of orthogonal modification of separate proteins in cell lysate. In addition, the concepts of proximity-driven catalysis are extended to include modification of the natural Fyn SH3 domain with metallopeptides based on a known proline-rich peptide ligand. The development of orthogonal catalyst-substrate pairs for modification in lysate, and the extension of these methods to new natural protein domains, highlight the capabilities for new reaction design possible in chemical approaches to site-specific protein modification.
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