期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 23, 页码 9565-9568出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja302290e
关键词
-
资金
- Office of Science, Office of Basic Energy Sciences, Division of Materials Sciences and Engineering, of the U.S. Department of Energy [DE-AC02-05CH11231]
Temperature is a key parameter in physiological processes, and probes able to detect small changes in local temperature are necessary for accurate and quantitative physical descriptions of cellular events. Several have recently emerged that offer excellent temperature sensitivity, spatial resolution, or cellular compatibility, but it has been challenging to realize all of these properties in a single construct. Here, we introduce a luminescent nanocrystal-based sensor that achieves this with a 2.4% change/degrees C ratiometric response over physiological temperatures in aqueous buffers, with a precision of at least 0.2 degrees C. Thermoresponsive dual emission is conferred by a Forster resonant energy transfer (FRET) process between CdSe-CdS quantum dot quantum rods (QD-QRs) as donors and cyanine dyes as acceptors, which are conjugated to QD-QRs using an amphiphilic polymer coating. The nanothermometers were delivered to live cells using a pH-responsive cationic polymer colloid, which served to both improve uptake and release nanocrystals from endosomal confinement. Within cells, they showed an unexpected enhancement in their temperature response and sensitivity, highlighting the need to calibrate these and similar probes within the cell.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据