期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 16, 页码 6102-6105出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja108404p
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资金
- Japan Society for the Promotion of Science
Click-free imaging of the nuclear localization of an alkyne-tagged cell proliferation probe, EdU, in living cells was achieved for the first time by means of Raman microscopy. The alkyne tag shows an intense Raman band in a cellular Raman-silent region that is free of interference from endogenous molecules. This approach may eliminate the need for click reactions in the detection of alkyne-labeled molecules.
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