期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 35, 页码 13836-13839出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja204775k
关键词
-
资金
- NIH [R01EB007689]
The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here transcription factor beacons, binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-kappa B), and measure binding activity directly in crude nuclear extracts.
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