期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 33, 页码 12960-12963出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja204781t
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资金
- Hoansha Foundation
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20117003, 23249004, 23113504]
- Grants-in-Aid for Scientific Research [23249004, 20117003] Funding Source: KAKEN
We identified a rhodol bearing a hydroxymethyl group (HMDER) as a suitable scaffold for designing fluorescence probes for various hydrolases. HMDER shows strong fluorescence at physiological pH, but phenolic O-alkylation of HMDER results in a strong preference for the spirocyclic form, which has weak fluorescence. As a proof of concept, we utilized this finding to develop a new fluorescence probe for beta-galactosidase. This probe has favorable characteristics for imaging in biological samples: it has good cellular permeability, and its hydrolysis product is well-retained intracellularly. It could rapidly and clearly visualize beta-galactosidase activity in cultured cells and in Drosophila melanogaster tissue, which has rarely been achieved with previously reported fluorescence probes.
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