期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 42, 页码 16970-16976出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja206324q
关键词
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资金
- DOD [BC061995]
- Gerald K. Branch Fellowship in Chemistry
- NSF
- UC [1 T32 GMO66698]
A new protein modification strategy has been developed that is based on an oxidative coupling reaction that targets electron-rich amino acids. This strategy relies on cerium(IV) ammonium nitrate (CAN) as an oxidation reagent and results in the coupling of tyrosine and tryptophan residues to phenylene diamine and anisidine derivatives. The methodology was first identified and characterized on peptides and small molecules, and was subsequently adapted for protein modification by determining appropriate buffer conditions. Using the optimized procedure, native and introduced solvent-accessible residues on proteins were selectively modified with polyethylene glycol (PEG) and small peptides. This unprecedented bioconjugation strategy targets these under-utilized amino acids with excellent chemoselectivity and affords good-to-high yields using low concentrations of the oxidant and coupling partners, short reaction times, and mild conditions.
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