期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 17, 页码 6745-6751出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja200225m
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan (Center of Education and Research for Chemical Biology of the Diseases) [20117003, 19205021, 22000006]
- Hoh-ansha Foundation
- Japanese Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [21500301, 20670002, 20117003] Funding Source: KAKEN
We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any washout process and is uniquely suitable for real-time imaging of protein dynamics on the cell surface. We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration.
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