期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 4, 页码 909-919出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja107847d
关键词
-
资金
- National Science Foundation [MCB-0918362]
- American Chemical Society [49968-DNI4]
- Welch Foundation [H-1683]
- Sealy and Smith Foundation
- W. M. Keck Foundation
- John S. Dunn Foundation
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [0918362] Funding Source: National Science Foundation
Despite their importance in macromolecular interactions and functions, the dynamics of lysine side-chain amino groups in proteins are not well understood. In this study, we have developed the methodology for the investigations of the dynamics of lisine NH3+ groups by NMR spectroscopy and computation. By using H-1-N-15 heteronuclear correlation experiments optimized for (NH3+)-N-15 moieties, we have analyzed the dynamic behavior of individual lysine NH3+ groups in human ubiquitin at 2 degrees C and pH 5. We modified the theoretical framework developed previously for CH3 groups and used it to analyze N-15 relaxation data for the NH3+ groups. For six lysine NH3+ groups out of seven in ubiquitin, we have determined model-free order parameters, correlation times for bond rotation, and reorientation of the symmetry axis occurring on a pico- to nanosecond time scale. From CPMG relaxation dispersion experiment for lysine NH3+ groups, slower dynamics occurring on a millisecond time scale have also been detected for Lys27. The NH3+ groups of Lys48, which plays a key role as the linkage site in ubiquitination for proteasomal degradation, was found to be highly mobile with the lowest order parameter among the six NH3+ groups analyzed by NMR. We compared the experimental order parameters for the lysine NH3+ groups with those from a 1 mu s molecular dynamics simulation in explicit solvent and found good agreement between the two. Furthermore, both the computer simulation and the experimental correlation times for the bond rotations of NH3+ groups suggest that their hydrogen bonding is highly dynamic with a subnanosecond lifetime. This study demonstrates the utility of combining NMR experiment and simulation for an in-depth characterization of the dynamics of these functionally most important side-chains of ubiquitin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据