期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 36, 页码 14208-14211出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja2057506
关键词
-
资金
- Kyoto University
- JSPS, Japan [22685018]
- MEXT, Japan
- Grants-in-Aid for Scientific Research [22685018, 21300162, 23659585] Funding Source: KAKEN
Monoamine inclose (MAO) A is a flavoenzyme that catalyzes the oxidation of biologically important monoamines and is thought to be associated with psychiatric disorders. Here, we report a strategy for rationally designing a F-19 magnetic resonance imaging probe for the specific detection of human MAO-A (hMAO-A) activity. Our designed F-19 probe was oxidized expeditiously by hMAO-A to produce 2-fluoro-4-nitrophenol via a spontaneous beta-elimination mechanism. Concomitant with the structural change of the probe to the product, the F-19 chemical shift changed by 4.2 ppm, which was enough to visualize the probe and enzymatic product separately. Importantly, our probe achieved excellent discrimination of hMAO-A from its isoform hMAO-B.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据