期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 40, 页码 16127-16135出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja206023e
关键词
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资金
- NIH/NIGMS [R01GM059907]
- U.S. National Science Foundation
Homogeneously glycosylated proteins are important targets for fundamental research and for biopharmaceutical development. The use of unnatural protein glycan linkages bearing structural similarity to their native counterparts can accelerate the synthesis of glycoengineered proteins. Here we report an approach toward generating homogeneously glycosylated proteins that involves chemical attachment of aminooxy glycans to recombinantly produced proteins via oxime linkages. We employed the recently introduced aldehyde tag method to obtain a recombinant protein with the aldehyde-bearing formylglycine residue at a specific site. Complex aminooxy glycans were synthesized using a new route that features N-pentenoyl hydroxamates as key intermediates that can be readily elaborated chemically and enzymatically. We demonstrated the method by constructing site-specifically glycosylated variants of the human growth hormone.
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