期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 5, 页码 1192-1195出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja108820s
关键词
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资金
- FCT, Portugal [SFRH/BPD/26991/2006, SFRH/BPD/30142/2006, SFRH/BDE/15501/2004]
- Fundação para a Ciência e a Tecnologia [SFRH/BDE/15501/2004, SFRH/BPD/30142/2006, SFRH/BPD/26991/2006] Funding Source: FCT
CORM-3, [fac-Ru(CO)(3)Cl(kappa(2)-H2NCH2-CO2)], is a well-known carbon monoxide releasing molecule (CORM) capable of delivering CO in vivo. Herein we show for the first time that the interactions of CORM-3 with proteins result in the loss of a chloride ion, glycinate, and one CO ligand. The rapid formation of stable adducts between the protein and the remaining cis-Ru-II(CO)(2) fragments was confirmed by Inductively Coupled Plasma-Atomic Emission Spectrocopy (ICP-AES), Liquid-Chromatography Mass Spectrometry (LC-MS), Infrared Spectroscopy (IR), and X-ray crystallography. Three Ru coordination sites are observed in the structure of hen egg white lysozyme crystals soaked with CORM-3. The site with highest Ru occupancy (80%) shows a fac-[(His15)Ru(CO)(2)(H2O)(3)] structure.
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