期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 6, 页码 1686-1689出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja109972p
关键词
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资金
- Burroughs Wellcome Career Award at the Scientific Interface
- National Institutes of Health [1-DP2-OD007078]
- William Bowes Jr. Stanford Graduate Fellowship
- Stanford Cardiovascular Institute
Although mechanical stress is known to profoundly influence the composition and structure of the extracellular matrix (ECM), the mechanisms by which this regulation occurs remain poorly understood. We used a single-molecule magnetic tweezers assay to study the effect of force on collagen proteolysis by matrix metalloproteinase-1 (MMP-1). Here we show that the application of similar to 10 pN in extensional force causes an similar to 100-fold increase in proteolysis rates. Our results support a mechanistic model in which the collagen triple helix unwinds prior to proteolysis. The data and resulting model predict that biologically relevant forces may increase localized ECM proteolysis, suggesting a possible role for mechanical force in the regulation of ECM remodeling.
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