期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 46, 页码 18554-18557出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja2080168
关键词
-
资金
- U.S. National Science Foundation [CHE-0809521]
- Ames Laboratory, U.S. Department of Energy, Office of Basic Energy Sciences [DE-AC02-07CH11358]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0809521] Funding Source: National Science Foundation
We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP- and glutathione-dependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据