期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 27, 页码 10368-10371出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja2036628
关键词
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资金
- NIH [GM68762, U54-CA112967, R01-EB010246, R01-GM081336]
- NSF
- DARPA
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (similar to 10-fold) reduced the time required to complete the assay and the sample volume used.
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