期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 19, 页码 7355-7357出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja2013278
关键词
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资金
- Grants Council of Hong Kong SAR, P. R. China [HKU7043/06P, HKU1/07C, HKU7042/07P, HKU7006/09P, HKU7049/09P, N_HKU752/09]
- Croucher Foundation
- Seed Funding Scheme for Basic Research of the University of Hong Kong
Bismuth-based drugs have long been used for the treatment of Helicobacter pylori infection. In this work, the metal content in H. pylori was monitored at the single-cell level by time-resolved inductively coupled plasma mass spectrometry, and similar to 2.9 x 10(7) Mg atoms/cell was determined for the wild-type. Bacteria treated with a Bi antiulcer drug deposited nearly 1.0 x 10(6) Bi atoms/cell, whereas the uptake process took similar to 3 h to reach the half-maximum. Interference of ferric ions on bismuth uptake was demonstrated, suggesting that the metallodrug can utilize certain iron-transport pathways in the pathogen. The approach provides a general strategy for monitoring metals in single cells, facilitating exploration of metal-relevant bioprocesses.
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