4.8 Article

Light-Activated Kinases Enable Temporal Dissection of Signaling Networks in Living Cells

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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 7, 页码 2124-2127

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AMER CHEMICAL SOC
DOI: 10.1021/ja1109979

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  1. Federation of European Biochemical Societies (FEBS)
  2. MRC [MC_UP_A024_1008, MC_U105181009] Funding Source: UKRI
  3. Medical Research Council [MC_U105181009, MC_UP_A024_1008] Funding Source: researchfish

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We report a general strategy for creating protein kinases in mammalian cells that are poised for very rapid activation by light. By photoactivating a caged version of MEK1, we demonstrate the specific, rapid, and receptor independent activation of an artificial subnetwork within the Raf/MEK/ERK pathway. Time-lapse microscopy allowed us to precisely characterize the kinetics of elementary steps in the signaling cascade and provided insight into adaptive feedback and rate-determining processes in the pathway.

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