期刊
PLOS ONE
卷 10, 期 4, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0123986
关键词
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资金
- National Special Fund for State Key Laboratory of Bioreactor Engineering of China [2060204]
- 973 Program [2013CB531200]
- NSFC [31225008, 91313301, 31071260, 31170815, 91013012]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100074110010]
- Shanghai Science and Technology Commission [12JC1402900, 11DZ2260600]
- Dawn Program of Shanghai Education Commission [11SG31]
- State Key Laboratory of Bioreactor Engineering
- 111 Project [B07023]
- Fundamental Research Funds for the Central Universities
Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo. NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.
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