期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 26, 页码 9972-9975出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja201361t
关键词
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资金
- Howard Hughes Medical Institute
- NIH/NIGMS [R01GM065865]
We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection.
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