期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 39, 页码 13575-13577出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja104493d
关键词
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资金
- F&M Hackman and Eyler funds [NSF-MCB-0448297]
- Research Corporation [CC6364]
- HHMI
- [NSF-DMR-09-69301]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [969301] Funding Source: National Science Foundation
Despite the importance of protein-polymer bioconjugates, there is no general method for producing homogeneous recombinant protein that contains polymer initiators at defined sites. To address this deficiency, we designed the amino acid 4-(2'-bromoisobutyramido)phenylalanine (1) as an initiator in atom-transfer radical polymerization (ATRP) that would provide a stable linkage between the protein and growing polymer. We synthesized 1 and evolved a Methanococcus jannaschii tyrosyl-tRNA synthetase/tRNA(CUA) pair to genetically encode this initiator in response to an amber codon. To demonstrate the utility of this initiator, we produced green fluorescent protein (GFP) with 1 site-specifically incorporated on its surface (GFP-1). Purified GFP-1 was then used as an initiator under standard ATRP conditions with a monomer, oligo(ethylene oxide) monomethyl ether methacrylate, efficiently producing a polymer-GFP bioconjugate where the polymer is connected at our selected site on GFP.
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