期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 24, 页码 8255-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja102932n
关键词
-
资金
- NSF [MCB 0843520]
- North Carolina Biotechnology Center
- National Institutes of Health
- [P41EB002031]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0843520] Funding Source: National Science Foundation
A general sequential assignment strategy for uniformly N-15-labeled uniaxially aligned membrane proteins is proposed. Mismatched Hartmann-Hahn magnetization transfer is employed to establish proton-mediated correlations among the neighboring N-15 backbone spins. Magnetically aligned Pf1 phage coat protein was used to illustrate the method. Exchanged and nonexchanged separated local field spectra were acquired and overlaid to distinguish the cross-peaks from the main peaks. Most of the original assignments from the literature were confirmed without selectively labeled samples. This method is applicable to proteins with arbitrary topology and will find use in assigning solid-state NMR spectra of oriented membrane proteins for their subsequent structure determination.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据