期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 24, 页码 8244-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja102257v
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资金
- Cancer Research Institute
- Starr Cancer Consortium
- Lerner Family
- NBC/NIH/NIAID [2 U54 AI057158-06]
Type III protein secretion is essential for many Gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.
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