期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 36, 页码 12552-12553出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja1055565
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资金
- National Institutes of Health
- Biomedical Technology Resource for NMR Molecular Imaging of Proteins
A mixture of phospholipids and Triton X-100 in a molar ratio of 5:1 forms well-aligned and stable bilayers that give superior solid-state NMR spectra of proteins. In a comparison, the oriented-sample (OS) solid-state NMR spectrum of Pf1 coat protein in aligned phospholipid bilayers displayed better resolution than the equivalent solution NMR spectrum of the same protein in micelles. Both samples and experimental parameters were fully optimized.
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