期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 43, 页码 15099-15101出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja1044192
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资金
- National Institute of General Medical Sciences [R01-GM086196]
Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of Halo Tag-based target-specific azido DCDHFs, a class of photoactivatable push pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.
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