期刊
PLOS ONE
卷 10, 期 6, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0130893
关键词
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资金
- Deutsche Forschungsgemeinschaft [NA429/2-1, SFB 1039-A05, SFB 1039-B04]
AMP-activated protein kinase (AMPK) maintains energy homeostasis by suppressing cellular ATP-consuming processes and activating catabolic, ATP-producing pathways such as fatty acid oxidation (FAO). The transcription factor peroxisome proliferator-activated receptor delta (PPAR delta) also affects fatty acid metabolism, stimulating the expression of genes involved in FAO. To question the interplay of AMPK and PPAR delta in human macrophages we transduced primary human macrophages with lentiviral particles encoding for the constitutively active AMPK alpha 1 catalytic subunit, followed by microarray expression analysis after treatment with the PPAR delta agonist GW501516. Microarray analysis showed that coactivation of AMPK and PPAR delta increased expression of FAO genes, which were validated by quantitative PCR. Induction of these FAO-associated genes was also observed upon infecting macrophages with an adenovirus coding for AMPK gamma 1 regulatory subunit carrying an activating R70Q mutation. The pharmacological AMPK activator A-769662 increased expression of several FAO genes in a PPAR delta- and AMPK-dependent manner. Although GW501516 significantly increased FAO and reduced the triglyceride amount in very low density lipoproteins (VLDL)-loaded foam cells, AMPK activation failed to potentiate this effect, suggesting that increased expression of fatty acid catabolic genes alone may be not sufficient to prevent macrophage lipid overload.
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