期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 27, 页码 9328-9334出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja100345z
关键词
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资金
- Cancer Research UK [C129/A10902]
- School of Pharmacy
- Fonds de la Recherche Scientifique-FNRS [CC. 1.5.286.09.F]
- University of Liege [FRSD-08/10]
- [MP0802]
We report on the self-assembled structures formed by 12-mer, 22-mer, and 45-mer telomeric RNA (teIRNA/TERRA) sequences compared to their DNA analogues, as studied by electrospray mass spectrometry, circular dichroism, and thermal denaturation. The major difference between telomeric RNA and DNA sequences is the ability of telomeric RNA to form higher-order dimeric assemblies, initiated by cation-mediated stacking of two parallel G-quadruplex subunits. The 5'-5' stacking had been observed recently by NMR for the r(GGGUUAGGGU) 10-mer (Martadinata, H.; Phan, A. T. J. Am. Chem. Soc. 2009, 131, 2570); the present work shows that stacking also occurs for the 22-mer containing four G-tracts and for the 45-mer containing eight G-tracts, suggesting a general structural feature of telomeric RNA. The importance of kinetic effects in multimer formation, unfolding, and structural rearrangements is also highlighted.
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