期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 132, 期 8, 页码 2585-2590出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja9074562
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资金
- ANR [PCV 07 1-0035]
- CNRS
- French Ministry of Research
- Universite de Strasbourg
Total synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which allows an efficient (>= 95%) and rapid (<15 mu s time-range) release of the fluorescent signal and displays remarkable two-photon uncaging cross sections (delta(a).Phi(u) = 3.7 GM at 740 nm). PENB-DDAO is cell permeable as demonstrated by the triggering of cytoplasmic red fluorescent signal in HeLa cells after one-photon irradiation (lambda(exc) around 360 nm) or by the generation of a red fluorescent signal in a delineated area of a single cell after two-photon photoactivation (lambda(exc) = 770 nm).
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