期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 131, 期 16, 页码 5792-5799出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja809554x
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资金
- NIH [CA 103823]
- National Institutes of Health [CA 125934-02]
- New York State Department of Health
- New York State Breast Cancer Research and Education Fund [C020919]
- Austrian Fonds zur Forderung der Wissenschaftlichen Forschung
A highly convergent synthesis of the sialic acid-rich biantennary N-linked glycan found in human glycoprotein hormones and its use in the synthesis of a fragment derived from the beta-domain of human Follicle-Stimulating Hormone (hFSH) are described. The synthesis highlights the use of the Sinay radical glycosidation protocol for the simultaneous installation of both biantennary side-chains of the dodecasaccharide as well as the use of glycal chemistry to construct the tetrasaccharide core in an efficient manner. The synthetic glycan was used to prepare the glycosylated 20-27aa domain of the beta-subunit of hFSH under a Lansbury aspartylation protocol. The proposed strategy for incorporating the prepared N-linked dodecasaccharide-containing 20-27aa domain into beta-hFSH subunit was validated in the context-of a model system, providing protected beta-hFSH subunit functionalized with chitobiose at positions 7 and 24.
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